Nano Engineering & Technology

Biography

Mr. Gautam received his master degree in Chemical Science from Institute of chemical technology (ICT), Mumbai, India. After completing his master degree he worked 2 years as Research Assistance at Aditya Birla Science and Technology Company. He is currently a pursuing his Ph.D. study at Institute of Chemistry, Academia Sinica, Taiwan.

 

Abstract

Nanotechnology-based cargo delivery to cells is a promising assay due to its efficiency and biocompatibility. Time-consuming and uncontrollable deliver amount are still thresholds in related research. In this study, we design a nanopillar-based platform for liposome delivery in vitro.

Thermoreponsive copolymer (p(NIPAm-co-AEMA)) is grafted from silicon nanowire (SINW) as a polymer nano brush to equip temperature-controllable liposome conjugations of the SINW. A liposome is introduced onto the polymer nano brush to form a nano-by-nano interface and is released through thermal-stimuli to generate a high local concentration for cellular uptake. Cryo-TEM images show that liposomes can attach to the polymer nano brush to form liposome-tethered nanopillars. Fluorescence quantifications suggest that up to 90% of the attached liposomes can be released with intactness. Furthermore, HEK 293T cell and calcein-loaded liposome are employed to investigate the cellular uptake kinetics. We found that the cellular uptake kinetic of HEK 293T cell is highly correlated to temperature stimulus in the nano-by-nano system. Fluorescence intensity difference quantified by flow cytometry indicate elevated cellular uptake efficiency, which is more than 10-fold increments at 4-hour incubation. Confocal images show that the liposomes stay at cytoplasm instead of lipid membrane after cellular uptake.